The aim of present study was to identify fatigue index by using repeated anaerobic effort among Handball, basketball, and volleyball players from Faculty of Physical Education of Sana’a University, and to identify the statistical significant differences of fatigue index between three games players. The descriptive approach was applied for its appropriateness to the nature of the study. The sample of the study consisted of (12) players, whom chosen intentionally, and classified into

 (3) Groups by (4) to each game. The selected sample practice regularly and take participation in competitions. A pilot study applied on (3) players by one from each. To evaluate RAST and FI all subjects completed multiple sprint running protocols. The design of the study requires participants to perform (6) sprints each of (35) meters with an interval of (10) sec of passive recovery between sprints.  After ending the RAST, results was calculated by using the following formula:

 Anaerobic power (watt) = weight x distance2 / time3

The results of this study showed an increase in the time of runs and increase in fatigue index from the first run until the sixth run.

 In spite of there were a significant differences in fatigue index in all three groups, but it was not high.

The researchers recommended giving more attention to anaerobic endurance (lactic endurance) of the players in order to delay fatigue.