The purpose of this study was to: (a) determine the effect of gender on preservice physical educators’ attitudes from Saudi Arabia (98 male) and United States (96 males, 48 females) toward teaching students with physical disability, autism, an intellectual disability, and emotional/behavioral disorders in the three areas of teaching, student learning, and academic preparation; and (b) determine the influence factors of gender, religion, culture, educational setting, and experience on the attitudes of undergraduate physical education students toward teaching students with disabilities. One way ANOVA was used in this study. Based on the results of the analyses, female participants from the United States showed more positive attitudes toward teaching students with specific disabilities than male participants in Saudi Arabia and United Sates. Females also showed a higher agreement in the affect of the gender factor on physical educators’ attitudes than both males’ participants from the two countries. In contrast, Saudi Arabian male participants significantly had more positive attitudes toward the importance of academic preparation area and the experience factor in influencing positively the physical educators’ attitudes toward the specific disabilities than female participants but not males.