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ملخص البحث

The purpose of this study was to investigate the bactericidal activity of copper (Cu, 10 ppm) and ascorbic acid (AA, 0.3% v/v) mixture against Staphylococcus aureus (S. aureus) ATCC 25923 (methicillin-susceptible) and ATCC 700698 (methicillin-resistant) strains. Approximately 5.5 log10 CFU/ml of each strain was individually inoculated into brain heart infusion (BHI) broth containing Cu, AA, and a Cu-AA mixture, and a time-kill assay was conducted to assess the bactericidal activity. In order to determine the efficacy of Cu-AA mixture as a surface decontaminating solution, the cells were first attached to glass slides (~6.5–7.5 log10 CFU per slide) and treated with a Cu-AA mixture for 1 min. The inner membrane permeability of the S. aureus strains was determined by measuring β-Galactosidase activity. Similarly, changes in the protein profile of treated bacterial cells were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The time-kill study revealed >3.0 log10 CFU/ml reduction in both strains within 24 h of incubation at 37 °C. Attached cells on glass slides were also reduced by > 3.0 log10 CFU per slide after 1 min of exposure to the Cu-AA mixture. The results indicate that the Cu-AA mixture was bactericidal against S. aureus strains and the damage to the inner cell membrane could be attributed to this effect.

المجال
Exercise Physiology
المؤلف
Rabin Gyawali; Tahl Zimmerman; Sulaiman O. Aljaloud; Salam A. Ibrahim
الناشر
Food ControlVolume 111,107062
سنة النشر
2020
بلد النشر
Saudi Arabia
اللغة
إنجليزي
الكلمات المفتاحية
Staphylococcus, aureus ,Copper ,Ascorbic, acid, Vitamin C, Decontaminating ,solution, Bactericidal, effect, Food ,safety
Arabic
Last updated on : January 12, 2023 1:13am